The Science Behind Dermal Night Repair with Retinol

Most recent scientific data have shown that Retinoids:

• INCREASE PGE-2 levels

• Block the ability of growth factors (like TGF) to stimulate collagen

• Stop the proliferation (growth) of fibroblasts

• Switch off collagen gene activity/collagen synthesis

• Halt the production of hyaluronic acid

• Inhibit the production of lysyl oxidase, the enzyme that crosslinks

collagen to elastin to form the elastic matrix critical to skin elasticity.

Will LORENOL® III + Sulforaphane help to Inhibit Inflammation in the presence of UVR?

There is only one safe way to do this, by combining Retinoids with anti-aging compounds that stimulate collagen production and block PGE-2. Recent clinical studies suggest that the combination of ingredients in the patented formula “LORENOL® III" can stimulate collagen and inhibit Cox-2 and PGE-2. Recent research has shown that redness from Retinoids are primarily due to one key inflammatory mediator, prostaglandin (PGE -2). This inflammatory mediator is one of the leading causes of sunburn and has been linked to cancer. It is also responsible for Inflammation, pain, poor wound healing, scarring, and hyperpigmentation.

Although Retinoids have shown significant results in reversing skin aging, they have also proven to increase PGE-2 levels dramatically. The graph displayed below shows test results on human keratinocytes (skin cells) grown in a culture that was treated with Retinol for up to 24 hours. These PGE -2 levels were measured every few hours during the study. Research indicates that Retinol increased PGE-2 levels almost four times in only two short hours. By the end of the 24 hours, this inflammatory mediator had risen to nearly eight times the original amount just by the introduction of retinoids. Because PGE-2 causes redness and produces symptoms mimicking a sunburn, it is easier to understand why topical retinoids can cause extreme irritation, redness, and sensitivity. It is also logical that UV Radiation from sunlight stimulates PGE-2 effects far above normal levels when combined with retinoids and sun exposure. For this very reason, Doctors strongly caution against sun exposure after applying any retinol products. (Sun exposure after retinol use can lead to severe skin damage, sunburn, and even skin sensitivity. By combining LORENOL’S® anti inflammatory effects with Retinol, it is possible to optimize the anti-aging benefits of Retinol without an increase in skin irritation, redness, and peeling.

The graphs below illustrate: Human skin study; cells were treated with Ultraviolet Radiation (UVR).

• The effects of the increase in PGE-2 levels (increase in skin “redness”)

• PGE-2 levels increase with UVR treatment

• Cells treated with Retinol increased the PGE-2 levels by more than two times those initially documented

• Cells treated with LORENOL® III + Sulforaphane did NOT show any increase in PGE-2 levels when exposed to UVR.

• The LORENOL® III + Sulforaphane compound appears to have completely prevented a major increase in PGE-2 in cells that were treated with Retinol and UVR.

• The compound appears to have wholly prevented a significant increase in PGE-2 in cells that were treated with Retinol and UVR.

Dermal Night Repair with Retinol Technical Study

Dermal Night Repair with Retinol is a proprietary blend of newly discovered, all-natural ingredients suspended in a luxurious non-silicone base, combined with the anti-aging compounds and a high concentration of medical-grade Retinols. Sulforaphane (Broccoli sprouts, the youngest of the broccoli plant). Sulforaphane first made the news in the late 1990s when researchers at the Johns Hopkins School of Medicine found that glucoraphanin, a potent anti￾cancer compound, was highly concentrated in tiny young broccoli sprouts. The anti-cancer actions were due primarily to Sulforaphane’s ability to block new blood vessel growth (angiogenesis) into tumors...causing them to shrink and die. - Broccoli Sprout Sulforaphane benefits beyond skin deep.

These three-day-old broccoli sprouts are naturally packed with as much as 100 times more glucoraphanin by weight than regular Broccoli Florets purchased in the grocery store. It is this concentrated glucoraphanin that, once transformed by naturally occurring enzymes in the skin into Sulforaphane, can block harmful inflammatory hormones that can lead to cancer. For generations, products containing Vitamin A (Retinol), Retinaldehyde, and Retinoic Acids have been used to treat signs of aging, albeit with very adverse side effects. Although Retinoids are very effective in treating aging skin, unfortunately, the side effects have been red, swollen, peeling, and sensitive skin. Lorenol Labs® newly discovered combination of compounds named “LORENOL® II” allows the benefits of medical￾grade Retinoids to be introduced into an anti-aging product WITHOUT the downside of:

• Redness

• Peeling

• Sun Sensitivity

By combining the anti-aging benefits of Retinol with our proprietary formula LORENOL® III, + sulforaphane, we have been able to increase the level of Retinol to 2.5% without any of the side effects that are typically associated with other Retinol-based products.

Vitamin A Chemistry Challenges

Although the benefits of retinoids have been used for decades, toxicity concerns, skin irritation, and photochemical instability have lessened the desire for their use. Retinoic acid, a topically applied ingredient, is recognized for its anti- aging benefits; it can be irritating to skin, however, and is sold as a prescription medication. Recently, natural derivatives and milder synthetics have become popular alternatives to retinoic acid. These derivatives become metabolized to the active form by skin cells. Recently, natural derivatives and milder synthetics have become popular alternatives to retinoic acid. These derivatives become metabolized to the active form by skin cells.

Retinol or vitamin A is one of the most popular topical retinoids currently being used, but its irritancy to the skin and its instability to sunlight have limited its scope and appeal. Retinol esters are quite often used to lower the irritation potential and increase stability, but a tradeoff is decreased retinoid activity and benefits. This novel retinoid product is the next-generation in anti-aging products, delivering the powerful performance of retinol and retinoid derivatives only with significantly lower irritation potential, thus supporting clear, visibly more youthful￾looking skin with better consumer acceptance. The mechanism of action of the Dermal Night Repair with Retinol is advanced compared to other retinol products. To interact with retinoid receptors, retinol must first be metabolized into more active forms, (Ex: retinaldehyde and retinoic acid use several enzymatic steps). One of the retinoids Lorenol Labs® uses is unique in that it processes innate retinoid activity, which binds directly to retinoid receptors without the need for metabolic breakdown to more biologically active forms. This novel retinoid product has been dermatologically tested to offer less irritation potential than regular retinol products and provides a gentle, safe, and effective anti-aging retinoid.

How Does LORENOL® Work?

LORENOL® is a newly discovered proprietary blend of all-natural ingredients found in foods and food flavoring agents that have been used for generations and which has been approved by ISO, the FDA, and classified as GRAS. This proprietary blend of natural ingredients has remarkable anti-inflammatory and anti- aging properties as well as the ability to help stimulate natural immune defenses. Recent studies have shown that LORENOL® has the capacity to stimulate collagen, elastin, and hyaluronic acid production in aging skin as well as the ability to inhibit harmful inflammatory mediators such as PGE- 2, IL-1, IL-8, LBT-4, and TNF-α.

Dermal Night Repair with Retinol has the added benefit of two very different and effective Retinoids. Studies have shown that using two distinctly different Retinoids, we can achieve optimal results in a much shorter period of time.

1. Hydroxypinacolone Retinoate has a higher molecular weight than Pro-Retinol. This allows the Retinol to penetrate the skin, while gradually, Pro-Retinol, with a much smaller amount of Retinol molecular weight, penetrates the skin more quickly.

2. Because Pro-Retinol moves into the surface of the skin at a much faster rate, it provides benefits almost immediately, whereas Hydroxypinacolone Retinoate, with its higher molecular weight, has a slower penetration and stays on the skin longer. This process offers continuous Retinol application to the skin over 24 hours, similar to a “time-release process,” and lengthens the exposure and the benefits of the Retinols.

3. This high amount of Retinol may not be easily tolerated in common topical skincare products. Because of the advantages of the compounds in LORENOL® III + Sulforaphane, however, Dermal Night Repair with Retinol can inhibit the inflammation caused by these Retinoids, while leaving the skin conditioned without the typical redness and sensitivity of other harsh retinol products. In short, this unique and highly effective anti-aging serum contributes to the skin-conditioning and stabilizes the look

of the skin when regularly applied.

4. Ongoing clinical studies have shown that LORENOL® III + Sulforaphane helps to improve dry, blemished, sensitive skin, even atopic dermatitis, without the skin sensitivities typically associated with high concentrations of Retinol. After only a few days of use, the result is younger, smoother, brighter, and more beautiful skin. Even those with the most sensitive skin will find this product is gentle enough to use nightly.

Cell proliferation and stimulating cell turnover are essential for normalizing cell renewal and the repair processes. As we age, skin becomes thinner and less elastic, leading to skin that is loose and sagging, with a more wrinkled appearance. Hydroxypinacolone Retinoate helps renew skin plumpness, elasticity, and hydration to provide a radiant and refreshed appearance. Moreover, this novel retinoid stimulates skin cell proliferation, restoring thickness to skin that has thinned over time. These processes can help fill in lines and wrinkles to give a more youthful appearance while safeguarding skin from further wrinkle development.

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